Table 1 Primary antibodies used for multiparametric staining for flow cytometry
From: The role of PB1-F2 in adaptation of high pathogenicity avian influenza virus H7N7 in chickens
Antigen | Clone | Conjugate | Manufacturer | Dilution |
|---|---|---|---|---|
AIV NP | HB65 | Â | In house | Â |
Aqua Zombie | Â | V500 | Biolegend | Â |
Chicken Bu-1 | AV20 | BV421 | Southern Biotech | 2 µg/mL |
Chicken CD25 | AV142 | pure | Bio-Rad AbD serotec | 1 µg/mL |
Chicken CD3 | CT-3 | BV421 | Southern Biotech | 5 µg/mL |
Chicken CD4 | Feb 35 | Pure | serotec | 1 µg/mL |
Chicken CD45 | LT40 | APC | Southern Biotech | 1 µg/mL |
Chicken CD8α | CT-8 | Biotin | Southern Biotech | 5 µg/mL |
Chicken CD8β | EP42 | pure | Southern Biotech | 0.625 µg/mL |
Chicken γδ TCR | TCR-1 | FITC | Southern Biotech | 5 µg/mL |
Chicken KUL-01 | KUL01 | Biotin | Southern Biotech | 5 µg/mL |
Chicken MHC-II | 2G11 | PE | Southern Biotech | 2 µg/mL |
Mouse aCas3 | C92-605 | PE | BD | Â |