Figure 1

bCaspase-1 is responsible for bGSDMD cleavage. HEK293T cells were co-transfected with plasmids encoding bCaspase-1 and bGSDMD for 24 h. A The supernatants were collected and analyzed for LDH release levels. B Microscopic images of the indicated HEK293T cells. Scale bar, 50 μm. C Cell lysates were analyzed with anti-Flag, anti-GFP, anti-Myc, and anti-GAPDH by Western blot. D Cell lysates were immunoprecipitated with anti-Myc antibody and then analyzed with anti-Flag and anti-Myc antibodies by Western blot. FL: full length; NT: N-terminal fragment; CT: C-terminal fragment. E Cells were incubated with Dil (a red fluorescent probe for cell membrane) and DAPI for 10 min. The fluorescent signals were observed with confocal immunofluorescence microscopy. * P < 0.05, ** P < 0.01, *** P < 0.001