Figure 8
From: A matter of differentiation: equine enteroids as a model for the in vivo intestinal epithelium
Morphological characterization of eqCE. A Representative brightfield images of eqCE cultivated with differently composed media at day 1, day 3 and day 5 after splitting. Spherical growth and extensive budding (black arrowheads) were observed in PM and DM1 and DM2, whereas in DM3 and DM4 the growth was mainly spherical. Scale bars: 100 µm. B Growth rates of eqCE cultivated with differently composed media after 4 days of cultivation. Means ± SEM; Mann–Whitney Rank Sum Test versus PM; Dots represent biological replicates; N = 6 (PM)/5 (DM1, DM2, DM4)/4 (DM3).