Figure 7

NCP BVDV-induced mitophagy restricts cGAS-mediated type I interferon signaling. A Western blot analysis of VDAC1 protein in cytoplasmic fractions from MDBK cells infected with NCP BVDV (MOI = 5) in the presence or absence of siParkin or siNC at 48 hpi. B MDBK cells were transfected with siParkin or siNC for 24 h. Then, the cells were infected with NCP BVDV (MOI = 5), qPCR assays were performed to determine the mtCO1 and mtND6 levels of mtDNA in cytoplasmic fractions isolated from the treated cells at 48 hpi. C MDBK cells were transfected with siParkin or siNC for 24 h. Then, the cells were infected with NCP BVDV (MOI = 5), Western blot assays were performed to determine the cGAS, p-cGAS, TBK1, p-TBK1, p-IRF3 and NS4B expression at 48 hpi. D MDBK cells were transfected with siParkin or siNC for 24 h. Then, the cells were infected with NCP BVDV (MOI = 5). RNA from each group was collected at 48 hpi, qRT-PCR assays were performed to determine the IFN-β expression in the indicated treatment cells. Data are given as means ± standard deviation (SD) from three independent experiments. P values were calculated using Student t test. An asterisk indicates a comparison with the indicated control. *P < 0.05; **P < 0.01; ns: not significant.