Figure 8

NCP BVDV-induced mitophagy restricts ROS-mediated inflammatory responses. A MDBK cells were transfected with siParkin or siNC for 24 h. Then, the cells were infected with NCP BVDV (MOI = 5). IFA analysis was performed to determine the mtROS levels in mock- and NCP BVDV-infected (MOI = 5) cells at 48 hpi. (Scale bar = 200 μm). The mean fluorescence intensity of mtROS was measured using the Image J software. B MDBK cells were transfected with siParkin or siNC for 24 h. Then, the cells were infected with NCP BVDV (MOI = 5). RNA from mock- and NCP BVDV-infected (MOI = 5) cells was collected at 48 hpi, then qRT-PCR assays were performed to determine the IL-1β and IL-18 expression in indicated treatment cells. Data are given as means ± standard deviation (SD) from three independent experiments. P values were calculated using Student t test. An asterisk indicates a comparison with the indicated control. *P < 0.05; **P < 0.01; ns: not significant.