Table 2 Primers used in this study
Primer | Sequence (5′–3′) | Target gene |
|---|---|---|
16S-F | GCGGAGCATGCGGATTA | 16S |
16S-R | AACGTGCTGGCAACATAGGG | Â |
Δphi456-F | TCAGGCCTATCCAGTAC | Deletion of phi456 |
Δphi456-R | CGGTATCGTATGACTGA | |
qPCR-phi456-F | TCGTGATAACACACGCAAC | Circular phi456 |
qPCR-phi456-R | TGAACCTGAAACAATGGAC | |
RT-fimH-F | CTTATGGCGGCGTGTTATCT | fimH |
RT-fimH-R | CGGCTTATCCGTTCTCGAATTA | |
RT-fimA-F | GTTGTTCTGTCGGCTCTGTC | fimA |
RT-fimA-R | TAAAGTGAACGGTCCCACCA | |
RT-ropS-F | AGAGTAACTTGCGTCTGGTGGTAAA | ropS |
RT-ropS-R | ATAGTACGGGTTTGGTTCATAATCG | |
RT-katE-F | GATCTTCTCGATCCAACCAAAC | katE |
RT-katE-R | CACCAAGACGACTGATTTGTGT | |
RT-oxyR-F | TGAGGTGAAAGTCCTTAAAGAGATG | oxyR |
RT-oxyR-R | GTCTGTGCTTCATGCAGATACATT | |
TLR3-F | AACGGAGTTGCAAGGGAATG | TLR3 |
TLR3-R | ATCAGGTCAGGTGCTCTCCC | |
TLR4-F | GCTCCTGGCTAGGACTCTGAT | TLR4 |
TLR4-R | GGCTAGCAGGAAAGGGTGTG | |
TLR9-F | CCTCTCTCCAAGCCCTACCT | TLR9 |
TLR9-R | CAGTCCTGGTTCTGAAGCCT | |
TNF-α-F | TCTCCTTCCTGATCGTGGCA | TNF-α |
TNF-α-R | GGAGAGTGGATGAAGGCTGG | |
TNF-β-F | CAGGAACCCAAGCATCCACC | TNF-β |
TNF-β-R | AGGAAGTGGGCACTGAACAA | |
β-Actin-F | GCTGTCCCTGTATGCCTC | β-Actin |
β-Actin-R | AGATGTCAGCGCACCAC |