Figure 3.
3Dpol facilitates DHAV-1 replication by enhancing viral RNA translation efficiency. A DEFs cells were transfected with 4 μg of pBudCE-3D or pBudCE4.1 vector, and then were inoculated with 104 copies of DHAV-1 at 48 hpt. The viral replication levels in both group were analyzed by Western blot using anti-DHAV-1 antiserum (1:100). B Viral protein levels in both groups were measured using ImageJ software, and the grayscale values corresponding to the bands of each protein between groups were conducted. The y-axis represents the ratio of the grayscale value of the viral protein to that of β-actin, whereas the x-axis represents the time, in hours post-infection. C qRT-PCR results indicating the number of viral copies in DEFs transfected with the pBudCE-3D or pBudCE4.1 vector. D Comparison of FLUC activities in DEFs transfected with the pBudCE-3D or pBudCE4.1 vector. *P < 0.05, ** P < 0.01, *** P < 0.001. Bars show the mean ± SD of three independent experiments (n = 3).