Figure 4

ILF2 negatively regulates DHAV-1 replication. A The silencing effect of ILF2 in DEFs at 60 hpt were measured through Western blot using anti-ILF2 antiserum (1:100). The Mock group indicated blank DEFs, the Control group indicated DEFs transfecting with negative control siRNA, and the siILF2 group indicated DEFs transfecting with siILF2. B qRT-PCR results of viral copies of DHAV-1 in control group and siILF2 group at 24, 36, 48, and 60 hpi. C Western blot analysis of the viral protein level of DHAV-1 in the siILF2 group and control group at 24, 36, 48, and 60 hpi. The anti-DHAV-1 antiserum (1:100) was used in this assay, and the gray scale value of each band was indicated D. E The expression levels of ILF2 in control group and EXILF2 group were detected by Western blot using anti-ILF2 antiserum (1:100) 48 h later. The Mock group indicated blank DEFs, the Control group indicated DEFs transfecting with pcDNA3.1/V5-His B vector, and the EXILF2 group indicated DEFs transfecting with pCDNA3.1-ILF2. F qRT-PCR results of viral copies of DHAV-1 in control group and EXILF2 group at 24, 36, 48, and 60 hpi. G The viral protein levels of DHAV-1 in the EXILF2 group and control group at 24, 36, 48, and 60 hpi were measured through Western blot using anti-DHAV-1 antiserum (1:100). H The grayscale value of each protein band in both groups was measured using ImageJ software. The y-axis represents the ratio of the gray value of the viral proteins to β-actin, while the x-axis represents the hours post-infection. * P < 0.05, ** P < 0.01, *** P < 0.001. Bars show the mean ± SD of three independent experiments (n = 3).