Skip to main content
Figure 5 | Veterinary Research

Figure 5

From: Interleukin-2 enhancer binding factor 2 negatively regulates the replication of duck hepatitis A virus type 1 by disrupting the RNA-dependent RNA polymerase activity of 3D polymerase

Figure 5

ILF2 suppresses DHAV-1 replication by disrupting the RdRP activity of 3Dpol. A Western blot results of the expression level of 3D and ILF2 in DEFs transfected with pBudCE-3D or pBudCE-3D-ILF2 using an anti-MYC (1:3000) or anti-ILF2 (1:100) antibody. B Western blot analysis of DHAV-1 viral replication levels in DEFs transfected with the pBudCE4.1 vector, pBudCE-3D, or pBudCE-3D-ILF2 using anti-DHAV-1 antiserum (1:100); the grayscale value of each band C. D The growth characteristics of DHAV-1 in DEFs transfected with the pBudCE4.1 vector, pBudCE-3D, or pBudCE-3D-ILF2 measured using qRT-PCR. E Comparison of FLUC activities in DEFs transfected with the pBudCE4.1 vector, pBudCE-3D, or pBudCE-3D-ILF2. * P < 0.05, ** P < 0.01, *** P < 0.001. Bars show the mean ± SD of three independent experiments (n = 3).

Back to article page

Veterinary Research

ISSN: 1297-9716