Table 1 Primers used in this research.
Primers | Sequence (5′—3)a | Purpose | Genebank Access No.b |
|---|---|---|---|
Primers for reverse transcription | Â | ||
 RT-ILF2-R | CTCCTGAGTCTCCATGCTCTCCTCTT | RT-PCR for ILF2 | XM_038167928 |
Primers for plasmids construction | Â | ||
 ILF2-EcoRI-F | ccgGAATTCGGATGGCTTTCCCGCGGGTTA | pCMV-myc-ILF2 | XM_038167928 |
 ILF2-XhoI-R | ccgCTCGAGTCACTCCTGAGTCTCCATGCTCT | ||
 ILF2-HindIII-F | cccAAGCTTGCCACCATGGCTTTCCCGCGGGTTA | pCDNA3.1-ILF2 | XM_038167928 |
 ILF2-BamHI-R | cgcGGATCCTCACTCCTGAGTCTCCATGCTCT | PBudCE-3D-ILF2 | |
 ILF2-BamHI-F | cgcGGATCCATGGCTTTCCCGCGGGTTA | pET32-ILF2 | XM_038167928 |
 ILF2-HindIII-R | cccAAGCTTTCACTCCTGAGTCTCCATGCTCT | ||
 3D-BglII-F | ggaAGATCTGGGAAAGTAGTAAGCAAGCAATA | pCMV-HA-3D | MW462237 |
 3D-NotI-R | aagGCGGCCGCTTAGATCATCATGCAAGC | ||
 3D-NotI-F  3D-BglII-R | aagGCGGCCGCGGGAAAGTAGTAAGCAAGCAATA ggaAGATCTTTAGATCATCATGCAAGC | PBudCE-3D | MW462237 |
 VP0-BamHI-F | cgcGGATCCATGGATACTCTTACCAAAAAC | pET32-vp0 | MW462237 |
 VP0-XhoI-R | ccgCTCGAGCTGATTGTCAAATGGTCGGGGACAC | ||
 3UTR-F | ACTGTTGGTCCGCAGGTACCATAAA | 3′ UTR of DHAV-1 | MW462237 |
 3UTR-R | AGGTAGGGTAGGGAATAGTAAA | ||
 3UTR-reverse-F | AGGTAGGGTAGGGAATAGTAAAG | Reverse sequence of 3′ UTR of DHAV-1 | MW462237 |
 3UTR-reverse-R | ACTGTTGGTCCGCAGGTACCATAAACC | ||
Primers for qRT-PCR | Â | ||
 ILF2-qRTPCR-F | GCAGACGCTTGTCCGCATCC | qRT-PCR for ILF2 | XM_038167928 |
 ILF2-qRTPCR-R | GGTTCCTCCTGGTTCTCCTCTTCC | ||
 DHAV-qRT-PCR-F | AAACACCCACTGGCTTTGGA | qRT-PCR for DHAV-1 | MW462237 |
 DHAV-qRT-PCR-R | GGTCCTCACGGAAAGTGGAG | ||
 GAPDH-F | ATGTTCzGTGATGGGTGTGAA | qRT-PCR for GAPDH | XM_061080759.1 |
 GAPDH-R | CTGTCTTCGTGTGTGGCTGT | ||
Primers for RNA interference | Â | ||
 siILF2 (sence) | UCAAUUUGCACCUCGAAAGUU | RNAi against ILF2 | XM_038167928 |
 siILF2 (antisence) | GGUAGCAGAACGUCCCACAGC | ||
 NC siRNA (sence) | UUCUCCGAACGUGUCACUUtt | Negative control siRNA | XM_038167928 |
 NC siRNA (antisence) | ACGUGACACGUUCGGAGAAtt | ||