Figure 3

Representation of the superchallenge experiment to investigate Staphylococcus chromogenes priming against Streptococcus uberis infection. Mice were intramammarily inoculated with either 10⁴ CFU S. chromogenes IM (challenge) or sham (PBS), followed by a second inoculation 24 h later with either 10³ CFU S. uberis NADC C-1 (infection) or sham (PBS). This led to 4 groups i.e., (1) S. chromogenes IM-S. uberis-inoculated (black), (2) S. chromogenes IM-sham (horizontal stripes), (3) sham-S. uberis (asterisks) and (4) sham-sham (vertical stripes) mice. “n” represents the number of animals inoculated in each group.